breathable film  (AutoMate Scientific Inc)

Journal: eLife

Article Title: eDNA-stimulated cell dispersion from Caulobacter crescentus biofilms upon oxygen limitation is dependent on a toxin–antitoxin system

doi: 10.7554/eLife.80808

Figure Lengend Snippet: ( A ) Representative growth curves of cultures grown in M2G medium at 30°C, followed by OD 600 over time. Cultures were grown under moderate aeration (no shaking, 1 ml culture in a 2-ml microtube sealed with AeraSeal breathable film), and under maximal aeration conditions (300 rpm shaking, 1 ml culture in a 15-ml culture tube). ( B ) Quantification of eDNA released in the planktonic phase in the same cultures, using PicoGreen. Results are given as the average of at least three independent experiments and the error bars represent the standard error of the mean (SEM).

Article Snippet: C. crescentus cultures were grown under four different aeration conditions: (1) maximal aeration by growing 1 ml of culture in a 15-ml glass culture tube tilted and shaken at 300 rpm; (2) high aeration with 1 ml of culture in a 2-ml microtube sealed with a 1.5 × 1.5 cm piece of sterile breathable sealing film (AeraSeal, Excel Scientific), to allow air exchange, in a shaking ThermoMixer (Eppendorf), at 500 rpm; (3) moderate aeration with 1 ml of a culture in a 2-ml microtube sealed with a 1.5 × 1.5 cm piece of sterile breathable sealing film (AeraSeal, Excel Scientific), to allow air exchange, in a non-shaking ThermoMixer (Eppendorf); and (4) limited aeration with 1 ml of a culture in a 2-ml microtube tightly sealed with an air tight rubber plug to prevent air exchange with the environment, in a non-shaking ThermoMixer (Eppendorf).

Techniques:

Journal: Current protocols in plant biology

Article Title: High-throughput yeast one-hybrid screens using a cell surface gLUC reporter

doi: 10.1002/cppb.20086

Figure Lengend Snippet: A) Basic protocol 1: mating TF-library and Y1H reporter strains in liquid medium. B) Basic protocol 2: quantification of gLUC59 activity in liquid cultured yeast cells. All illustrated microplates are 384-well and the text below each one describes its content (a star indicates that a deep-well microplate is used). Arrows indicate protocol incubation steps (red), liquid handling steps (blue) and data processing steps (black). Dotted- or full-lined arrows indicate protocol steps that maintain or introduce a new microplate, respectively. Text next to each arrow provides a brief step description. Procedure timeline is indicated on the left side of each panel.

Article Snippet: Breathable microplate sealing film ( e.g. AeraSeal, Excel Scientific, cat B-100).

Techniques: Activity Assay, Cell Culture, Incubation, Introduce